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Ruthstein Lab

Biomolecular EPR spectroscopy.

In our lab, we utilize EPR measurements together with NMR, CD, various biochemical experiments, and computational methods to study in detail the cellular copper cycle in eukaryotic and prokaryotic systems.

All proteins are expressed and purified in our lab.


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The Lab Research.


Our research group uses biophysical, biochemical, and spectroscopic tools to resolve the copper transfer mechanism in eukaryotic and prokaryotic systems.

Given the ubiquity of metal’s presence in the cells, it follows that even small errors in the way biometals are regulated are likely to impact cell function and survival. It is thus tremendously important to understand each little step in the copper cycle in order to build a fundamental understanding of sources of the disruption of copper homeostasis. 


Hirsch, M.; Hofmann, L. Shenberger, Y.; Gevorkyan-airapetov, L.; Ruthstein, S.; Conformations and local dynamics of the CopY metal sensor revealed by EPR spectroscopy. Biochem. 2023.


Shenberger, Y.; Gevorkyan-irapetov, L.; Hirsch, M.; Hofmann, L.; Ruthstein, S. An in-cell spin-labelling methodology provides structural information on cytoplasmic proteins in bacteria. Chem. Comm. 2023.


Yasin, A.; Mandato, A.; Hofmann, L.; Igbaria-Jaber, Y.; Shenberger, Y.; Gevorkyan-Airapetov, L.; Saxena, S.; Ruthstein, S.; The dynamic plasticity of P. aerguinosa CueR copper transcription factor upon cofactor and DNA bindingChemBioChem. 2024.




Aug. 2023

Congratulations to Yulia and Lada for their accepted paper to Chem. Comm.


May 2024

Congratulations to Ameer for his 1st author publication in ChemBioChem. 

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