Biomolecular EPR spectroscopy.
In our lab, we utilize EPR measurements together with NMR, CD, various biochemical experiments, and computational methods to study in detail the cellular copper cycle in eukaryotic and prokaryotic systems.
All proteins are expressed and purified in our lab.
The Lab Research.
Our research group uses biophysical, biochemical, and spectroscopic tools to resolve the copper transfer mechanism in eukaryotic and prokaryotic systems.
Given the ubiquity of metal’s presence in the cells, it follows that even small errors in the way biometals are regulated are likely to impact cell function and survival. It is thus tremendously important to understand each little step in the copper cycle in order to build a fundamental understanding of sources of the disruption of copper homeostasis.
Sameach, H.; Ghosh, S.; Gevorkyan-Aiapetov, L.; Saxena, S.; Ruthstein, S.; EPR Spectroscopy detects various active state conformations of the transcriptional regulator CueR. Angew. Chem. Int. Ed. 2019, 58, 3053-3056.
Perkal, O.; Qasem, Z.; Turgeman, M.; Schwartz, R.; Gevorkyan-Airapetov, L.; Pavlin, M.; Magistrato, A.; Major, D.; Ruthstein, S.; Cu(I) controls conformational states in the human metallochaperone Atox1: an EPR and multiscale simulation study. J. Phys. Chem. B. 2020, 124, 4399-4411.
Congratulations to Gulshan, Shelly, Yulia and Lada for their accepted paper on ATSM-Cu(II) uptake in cancer cells.
Congratulations to Lukas and Melanie for their accepted review on copper homeostasis in PA.