Ruthstein Lab
Biomolecular EPR spectroscopy.
In our lab, we utilize EPR measurements together with NMR, CD, various biochemical experiments, and computational methods to study in detail the cellular copper cycle in eukaryotic and prokaryotic systems.
All proteins are expressed and purified in our lab.
For more information about the lab, pls click
The Lab Research.
Our research group uses biophysical, biochemical, and spectroscopic tools to resolve the copper transfer mechanism in eukaryotic and prokaryotic systems.
Given the ubiquity of metal’s presence in the cells, it follows that even small errors in the way biometals are regulated are likely to impact cell function and survival. It is thus tremendously important to understand each little step in the copper cycle in order to build a fundamental understanding of sources of the disruption of copper homeostasis.
Meron, S.; Peleg, S.; Shenberger, Y.; Hofmann, L.; Gevorkyan-airapetov, L.; Ruthstein, S.; Tracking disordered extracellular domains of membrane proteins in the cell with Cu(II)-based spin-labels. JPC B. 2024
Shenberger, Y.; Gevorkyan-irapetov, L.; Hirsch, M.; Hofmann, L.; Ruthstein, S. An in-cell spin-labelling methodology provides structural information on cytoplasmic proteins in bacteria. Chem. Comm. 2023.
Yasin, A.; Mandato, A.; Hofmann, L.; Igbaria-Jaber, Y.; Shenberger, Y.; Gevorkyan-Airapetov, L.; Saxena, S.; Ruthstein, S.; The dynamic plasticity of P. aerguinosa CueR copper transcription factor upon cofactor and DNA binding. ChemBioChem. 2024.
News!
Jun. 2024,
Congratulations to Melanie and Lukas for their accepted paper to Biophys. Reports.
Aug 2024,
Congratulations to Shelly and Shahaf for their accepted paper to JPC B.